烈冰生信云平臺(tái)助力單細(xì)胞測序半監(jiān)督深度學(xué)習(xí)算法DISC——推測dropouts基因結(jié)構(gòu)和表達(dá)

單細(xì)胞轉(zhuǎn)錄組測序在單細(xì)胞層面對(duì)轉(zhuǎn)錄組進(jìn)行測序與分析，現(xiàn)正被廣泛應(yīng)用于分析細(xì)胞的異質(zhì)性與多樣性。但是，過多的假零表達(dá)一直是困擾單細(xì)胞轉(zhuǎn)錄組數(shù)據(jù)分析的問題之一，該問題被稱為dropouts，它會(huì)扭曲基因的表達(dá)分布，并造成細(xì)胞類型分類的錯(cuò)誤。而且，由于近年來單細(xì)胞測序技術(shù)與產(chǎn)業(yè)迅速發(fā)展，單次單細(xì)胞測序?qū)嶒?yàn)的通量已從數(shù)千個(gè)細(xì)胞大幅提升到百萬級(jí)的細(xì)胞數(shù)量，而對(duì)于單個(gè)細(xì)胞的測序深度則較淺，這使得dropout

烈冰助力||RNA-seq揭示老化有機(jī)氯農(nóng)藥帶來的長期環(huán)境風(fēng)險(xiǎn)

原文鏈接：https://doi.org/10.1016/j.envint.2020.105778 往期回顧：空間轉(zhuǎn)錄組樣本不合格？細(xì)節(jié)決定成敗，烈冰送您最詳細(xì)的空轉(zhuǎn)樣本準(zhǔn)備指南空間轉(zhuǎn)錄組||烈冰生物關(guān)鍵技術(shù)——冷凍切片制備技術(shù)干貨||揭秘空間轉(zhuǎn)錄組實(shí)驗(yàn)核心步驟——掃描成像和組織透化優(yōu)化單細(xì)胞測序在生命科學(xué)領(lǐng)域的應(yīng)用研討會(huì)精彩

烈冰助力||單細(xì)胞RNA測序鑒定半月板祖細(xì)胞并揭示半月板退變機(jī)制

半月板在關(guān)節(jié)穩(wěn)定、減震、接觸力分布和關(guān)節(jié)潤滑等方面起著重要作用，其包含不同的細(xì)胞類型，主要包括軟骨細(xì)胞形態(tài)細(xì)胞和纖維細(xì)胞形態(tài)細(xì)胞。然而，半月板的細(xì)胞類型組成、分布和相應(yīng)的生物標(biāo)志物，以及治療半月板退變的生物靶點(diǎn)仍不清楚。 2019年12月7日，中山大學(xué)附屬第一醫(yī)院廖威明教授課題組和張志奇教授課題組通過ScRNA-Seq詳細(xì)描繪半月板的細(xì)胞組成，鑒定出一種在半月板組織工程中具有應(yīng)用前景的祖細(xì)胞，并證

烈冰助力||16s rRNA測序揭示神經(jīng)肽Y1受體拮抗劑調(diào)節(jié)大鼠腸道菌群并緩解骨質(zhì)疏松癥

絕經(jīng)后骨質(zhì)疏松癥(Postmenopausal osteoporosis ，PMO)是最常見的原發(fā)性骨質(zhì)疏松癥，是由于絕經(jīng)后雌激素缺乏導(dǎo)致的骨強(qiáng)度降低和骨脆性增加。腸道菌群與骨代謝密切相關(guān)，腸道細(xì)菌過度生長的患者骨密度明顯降低。而神經(jīng)肽Y（Neuropeptide Y，NPY）可以通過Y1受體(Y1R)對(duì)腸道直接起到抗菌的作用。那么，NPY能否通過調(diào)節(jié)大鼠腸道菌群，發(fā)揮抗骨質(zhì)疏松作用呢？

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葡萄寒冷應(yīng)激的轉(zhuǎn)錄組測序研究-寧夏大學(xué)(用了ASD)

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蓮花SSR分子標(biāo)記開發(fā)-中國科學(xué)院陳山植物園

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